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Sweet diterpenic glycosides balance of a new cultivar of Stevia rebaudiana (Bert.) Bertoni: Isolation and quantitative distribution by chromatographic, spectroscopic, and electrophoretic methods

Paper ID Volume ID Publish Year Pages File Format Full-Text
36519 45134 2005 8 PDF Available
Title
Sweet diterpenic glycosides balance of a new cultivar of Stevia rebaudiana (Bert.) Bertoni: Isolation and quantitative distribution by chromatographic, spectroscopic, and electrophoretic methods
Abstract

A dichloromethane fraction and a ultrafiltered water extract of leaves of a new cultivar of Stevia rebaudiana were submitted to separated downstreaming fractionation in silica gel columns. Non-glycosylated and glycosylated pairs for stigmasterol and β-sistosterol were obtained from the former. Stevioside and rebaudioside-A were resolved from the second hydrophilic extract. The structures of these natural compounds were then confirmed through gas chromatography coupled to mass spectrometry and hydrogen/carbon nuclear magnetic resonance. The main sweetener glycoside pair, following scaled-up water extraction, and sugar derivatives downstreaming by membrane filtration and ionic resin exchange, was analyzed by thin layer densitometered chromatography, high-pressure liquid chromatography, and capillary electrophoresis. Thus, an unusual sweetener ratio on the behalf of rebaudioside-A, a glycoside almost devoid of bitter after-taste, was found for a new Stevia cultivar (UEM-320) as compared to other native cultivars, where the bitterer stevioside was the major diterpenic glycoside.

Keywords
Stevia rebaudiana; Stevioside; Rebaudioside-A; Sweeteners; TLC; HPLC; CE
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Sweet diterpenic glycosides balance of a new cultivar of Stevia rebaudiana (Bert.) Bertoni: Isolation and quantitative distribution by chromatographic, spectroscopic, and electrophoretic methods
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 40, Issue 11, November 2005, Pages 3587–3594
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us