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Decolorization of some synthetic dyes using optimized culture broth of laccase producing ascomycete Paraconiothyrium variabile

Paper ID Volume ID Publish Year Pages File Format Full-Text
3654 181 2012 7 PDF Available
Title
Decolorization of some synthetic dyes using optimized culture broth of laccase producing ascomycete Paraconiothyrium variabile
Abstract

Optimization of the medium components for production of laccase by Paraconiothyrium variabile using response surface methodology was investigated. Initial screening by Plackett–Burman design was performed to select major variables out of eleven medium components, among which peptone, CuSO4, and xylidine were found to have significant effects on laccase production. After application of the steepest ascent to approach the proximity of the optimum point, a central composite design was employed to optimize the level of the selected variables. In optimum concentrations of the most effective parameters, including peptone, 2.2 g/L, CuSO4, 0.03 g/L, and xylidine 1.29 mM, extracellular laccase activity was enhanced from 970 U/L (in basal medium) to 16,678 U/L, which means a 17-fold increase in laccase production in the optimized medium. Supernatant of the optimized medium was used for decolorization of five synthetic dyes, among which 93% of Remazol brilliant blue R (with initial concentration of 600 mg/L) disappeared after 3 h treatment in the presence of 5 mM hydroxybenzotriazole.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Medium for submerge culture of Paraconiothyrium variabile was optimized for laccase production. ► Optimization experiments increased laccase production from 970 U/L to 16,678 U/L. ► The optimized medium decolorized five tested dyes during 180 min incubation.

Keywords
Paraconiothyrium variabile; Laccase; Enzyme production; Submerged culture; Enzyme biocatalysis; Waste treatment
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Decolorization of some synthetic dyes using optimized culture broth of laccase producing ascomycete Paraconiothyrium variabile
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 60, 15 January 2012, Pages 9–15
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us