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Application of down flow jet loop bioreactors in implementation and kinetic determination of solid–liquid enzyme reactions

Paper ID Volume ID Publish Year Pages File Format Full-Text
36549 45136 2005 6 PDF Available
Title
Application of down flow jet loop bioreactors in implementation and kinetic determination of solid–liquid enzyme reactions
Abstract

Glucose isomerization to fructose by immobilized glucose isomerase as an example of solid–liquid enzyme reaction has been studied in a down flow jet loop reactor (DJR). Conversion of glucose to fructose has been measured and compared with that formed in a batch reactor. The performance capability of the DJR was observed to be higher than that of the conventional reactors. The optimum operating conditions of the reaction system have been also determined using the Taguchi experimental design method. The effects of enzyme loading, draft tube diameter, position of nozzle inside the draft tube, and gas and liquid flow rates on glucose conversion have been considered. Analysis of the results revealed that the draft tube diameter has the highest and the ratio of liquid circulation rate to the gas flow rate has the least effects on the conversion of glucose. It was found that the experimental data could be correlated favoarably with a modified structure of the Michalis–Menten model consisting of two transition complexes.

Keywords
Glucose isomerase; Fructose; Enzyme reactions; Down flow jet loop reactor; Immobilized enzyme
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Application of down flow jet loop bioreactors in implementation and kinetic determination of solid–liquid enzyme reactions
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 40, Issue 7, June 2005, Pages 2455–2460
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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