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Hydrolysis of starch by amylase from Bacillus sp. KCA102: a statistical approach

Paper ID Volume ID Publish Year Pages File Format Full-Text
36556 45136 2005 9 PDF Available
Title
Hydrolysis of starch by amylase from Bacillus sp. KCA102: a statistical approach
Abstract

Bacillus sp. KCA102 capable of producing thermostable amylase in a short fermentation time (∼18–20 h) has been isolated from a soil sample. The performance of amylase during hydrolysis of starch has been studied using response surface methodology. The parameters under study have been categorized into two, viz., physical parameters (pH, temperature and time) and chemical parameters (amounts of substrate and enzyme). The optimal conditions of pH, temperature and time at which maximum amount of glucose formed were found to be 7.1, 57.5 °C and 25 min, respectively. The optimal amounts of substrate and enzyme for maximum hydrolysis were found to be 0.5% starch and 94 mU/ml of amylase, respectively.

Keywords
Amylase; Hydrolysis; Response surface methodology; Central composite design; Physical parameters; Chemical parameters
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Hydrolysis of starch by amylase from Bacillus sp. KCA102: a statistical approach
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 40, Issue 7, June 2005, Pages 2499–2507
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
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Full-text PDF Download
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