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Use of dansyl N-acetyl glucosamine as substrate for chitin synthetase activities

Paper ID Volume ID Publish Year Pages File Format Full-Text
36559 45136 2005 7 PDF Available
Title
Use of dansyl N-acetyl glucosamine as substrate for chitin synthetase activities
Abstract

The biophysical properties of fungal cell walls are determined by the composition, level and structural arrangement of their polysaccharides. A small amount of glucosamine in the cell wall (1–3%) forms chitin, which is a β(1 → 4) linear homopolymer of N-acetylglucosamine (GlcNAc). Chitin synthetase activity is often analysed by radioactivity using [14C] GlcNAc. In this study, a fluorescent method was developed for assessing chitin synthetase activity using N-dansyl N-acetyl glucosamine as chitin synthetase substrate. The technique is based on the preparation of the fluorophore N-acetyl glucosamine and its subsequent decrease due to its polymerisation by chitin synthetase. The resulting decrease of fluorescence can be measured by spectrofluorometery. This method is suitable and adaptable for chitin synthetase assays. Results are parallel to those obtained by radioactivity method described in literature.

Keywords
Chitin synthetase; Dansyl N-acetyl glucosamine; Yeast cell walls
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Use of dansyl N-acetyl glucosamine as substrate for chitin synthetase activities
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 40, Issue 7, June 2005, Pages 2523–2529
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us