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Enhancement of cordycepin production in submerged cultures of Cordyceps militaris by addition of ferrous sulfate

Paper ID Volume ID Publish Year Pages File Format Full-Text
3657 181 2012 6 PDF Available
Title
Enhancement of cordycepin production in submerged cultures of Cordyceps militaris by addition of ferrous sulfate
Abstract

The effect of ferrous sulfate addition on production of cordycepin (3′-deoxyadenosine), a useful bioactive product with some pharmacological activities, was investigated in submerged cultures of Cordyceps militaris in shake flasks. The results showed that the optimal addition condition was on day 0 with 1 g/L of ferrous sulfate, and the maximal amount of cordycepin reached 596.59 ± 85.5 mg/L, about 70% higher than the control without ferrous sulfate addition. Meanwhile, the consumption of inosine 5′-monophosphate (IMP), a potential precursor of cordycepin, was decreased rapidly. Transcription levels of important genes encoding adenylosuccinate synthetase (purA), IMP cyclohydrolase (purH) and IMP dehydrogenase (guaB) in the purine nucleotide biosynthetic pathway were also studied. Compared to the control, the transcription level of purA was significantly up-regulated in ferrous sulfate supplemented cultures, while purH and guaB were slightly down-regulated. This work indicated that ferrous sulfate addition was a simple and useful strategy for improving cordycepin production, and the related information might be helpful for further manipulation and understanding of the cordycepin biosynthesis.

► Ferrous sulfate addition was found useful to enhance the cordycepin production. ► The related gene expression was studied for better manipulation of the cordycepin biosynthesis. ► The relationship between IMP content and cordycepin production was studied. ► purA gene expression was found important in cordycepin synthesis.

Keywords
Metabolite production; Submerged culture; Physiology; Medicinal mushroom; Cordyceps militaris; Gene transcription
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Enhancement of cordycepin production in submerged cultures of Cordyceps militaris by addition of ferrous sulfate
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 60, 15 January 2012, Pages 30–35
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us