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Statistical media optimization for cell growth and azadirachtin production in Azadirachta indica (A. Juss) suspension cultures

Paper ID Volume ID Publish Year Pages File Format Full-Text
36590 45137 2005 6 PDF Available
Title
Statistical media optimization for cell growth and azadirachtin production in Azadirachta indica (A. Juss) suspension cultures
Abstract

Azadirachtin is one of the most widely used biopesticide originating from a plant source. Its production from plant cell cultivation was viewed to overcome constraints associated with its regular supply from the seed kernels. In order to select the effective carbon and nitrogen source, different concentrations of carbon (sucrose/glucose) and nitrogen (NO3−/NH4+ ratio) were studied in A. indica suspension culture. Glucose turned out to be a better carbon source over sucrose yielding high biomass (6.32 g/L) and azadirachtin (11.12 mg/L) content. Nitrate alone as nitrogen source was favorable for both biomass and azadirachtin accumulation. Plackett–Burman design was adopted to select the most important nutrients influencing the growth and azadirachtin accumulation in suspension culture. After identifying effective nutrients, Central Composite Design (CCD) was used to develop mathematical model equations, study responses and establish the optimum concentrations of the key nutrients for higher growth and azadirachtin production. A maximum of 15.02 g/L biomass and 2.98 mg/g azadirachtin was produced using optimum nutrient concentrations representing 99 and 96% validity of the model prediction with respect to biomass and azadirachtin, respectively. This optimized media can be used for cultivation of A. indica cells in bioreactor for mass production of azadirachtin.

Keywords
Biopesticide; Azadirachtin; Suspension culture; Plackett–Burman; Central composite design
First Page Preview
Statistical media optimization for cell growth and azadirachtin production in Azadirachta indica (A. Juss) suspension cultures
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 40, Issue 12, December 2005, Pages 3795–3800
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering