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Covalent immobilization of triacylglycerol lipase onto functionalized nanoscale SiO2 spheres

Paper ID Volume ID Publish Year Pages File Format Full-Text
36601 45138 2006 8 PDF Available
Title
Covalent immobilization of triacylglycerol lipase onto functionalized nanoscale SiO2 spheres
Abstract

Three novel nano-silica dioxide (NSD) carriers with reactive aldehyde groups, i.e. NSD-1, NSD-2 and NSD-3, were firstly prepared through functionalization of the NSD material, and porcine pancreas lipase (PPL) was immobilized on the resulting carriers by using covalent coupling method. The activity recovery of immobilized PPL based on the carrier NSD-1, NSD-2 and NSD-3 reaches to 73%, 79% and 118%, respectively, and the values of apparent KM of them were all higher than that of the free PPL. The optimal pH and temperature region of the immobilized PPLs for the hydrolysis of olive oil were at 8.0 and 50–60 °C. Meanwhile, the immobilized PPLs have a good stability, adaptability and reusability.

Keywords
Nano-silica dioxide; Functionalization; Immobilized enzyme; Activity recovery; Polymerization; Space-arms
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Covalent immobilization of triacylglycerol lipase onto functionalized nanoscale SiO2 spheres
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 4, April 2006, Pages 770–777
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
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