fulltext.study @t Gmail

Virulent gene based DNA probe for the detection of pathogenic Bacillus cereus strains found in food

Paper ID Volume ID Publish Year Pages File Format Full-Text
36603 45138 2006 6 PDF Available
Title
Virulent gene based DNA probe for the detection of pathogenic Bacillus cereus strains found in food
Abstract

Development of DNA probe for detection of food borne pathogen is an emerging field. Bacillus cereus strain BIS59 isolated from radiorised shrimps contains plasmid, which is 21 kb in size and is responsible for the production of non-hemolytic toxin. In this study, the detection of plasmid in B. cereus provides a basis to develop the probe for detection of B. cereus. The restriction digestion of this isolated plasmid with EcoRI enzyme revealed multiple bands. This provided a useful way to construct a plasmid library originated from the B. cereus. The studies on restriction digestion with different restriction enzymes (HindIII, NcoI, NdeIII, KpnI, BclI, BamHI, HaeIII, HpaII, XbaI and SacI) have shown insufficient fragments which were unsuitable for probe development. In order to develop the probe and to detect toxic gene, a 3 kb size fragment obtained from the B. cereus plasmid using BglII restriction enzyme. Validity of the probe was evaluated by using 43 bacterial cultures. The sensitivity of the probe was verified including standard Bacillus species and food isolates of other bacterial species by autoradiography. All the pathogenic Bacillus species were found to be hybridizing with the probe. This toxin gene based DNA probe showed high specificity to identify B. cereus.

Keywords
Bacillus cereus; Non-hemolytic toxin; DNA probe; Hybridization; Radiolabeling and autoradiography
First Page Preview
Virulent gene based DNA probe for the detection of pathogenic Bacillus cereus strains found in food
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 4, April 2006, Pages 783–788
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us