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Incorporation of pepstatin into culture media for reduction of protease activity in filtrates from Aspergillus niger NRRL-3

Paper ID Volume ID Publish Year Pages File Format Full-Text
36604 45138 2006 5 PDF Available
Title
Incorporation of pepstatin into culture media for reduction of protease activity in filtrates from Aspergillus niger NRRL-3
Abstract

Aspergillus species have been used in different industrial applications including extracellular enzyme production and as potential hosts for recombinant heterologous proteins production because of their excellent growth and fermentation properties. However, recombinant protein production levels are typically low due to the impact of Aspergillus proteases in modifying and degrading the heterologous proteins, especially in the extracellular medium. The potential of using the protease inhibitor pepstatin in filamentous Aspergillus niger culture was investigated in this study. The majority of protease activity was released into the medium as biomass content decreases after maximum biomass concentration was attained and a low concentration of pepstatin effectively inhibited the extracellular protease activity to an extent of 80–90%. The effectiveness of pepstatin in inhibiting the majority of protease activity was further demonstrated in media supplemented with both non-peptide and peptide-containing nitrogen sources. Pepstatin addition had no effect on microbial growth. Dilution of the culture filtrate released inhibition, indicating that pepstatin did not have a notable effect on protease production and that inhibition was reversible.

Keywords
Aspergillus niger; Aspartyl protease; Pepstatin; Culture medium; Inhibition
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Incorporation of pepstatin into culture media for reduction of protease activity in filtrates from Aspergillus niger NRRL-3
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 4, April 2006, Pages 789–793
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us