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Evaluation of a biosensor assay to quantify polyclonal IgG in ovine serum used for the production of biotherapeutic antibody fragments

Paper ID Volume ID Publish Year Pages File Format Full-Text
36612 45138 2006 6 PDF Available
Title
Evaluation of a biosensor assay to quantify polyclonal IgG in ovine serum used for the production of biotherapeutic antibody fragments
Abstract

Here, we evaluate an automated, direct binding surface plasmon resonance (Biacore® C) biosensor assay for the determination of total IgG in hyper-immunised ovine serum for use within the biopharmaceutical industry for production of polyclonal Fab fragments, including the FDA approved polyclonal biotherapeutic CroFab™ (Crotalidae Polyvalent Immune Fab). Serum IgG concentrations were evaluated using recombinant Streptococcus protein G immobilised to the carboxymethyl dextran matrix of a CM5 sensor chip. Ligand immobilisation, sensor surface regeneration and ovine IgG binding responses were evaluated and the suitability of the method for the screening of large numbers of ovine serum samples within a quality control analytical laboratory discussed. The report describes the evaluation and application of a simple, sensitive and rapid assay for determination of total ovine polyclonal IgG that may also be applicable for the determination of antibody concentration within serum or crude supernatants produced by recombinant or transgenic technologies used for biotherapeutic antibody production.

Keywords
CM5, carboxymethylated dextran coated biosensor chip (Biacore®); CV, coefficient of variation; EDC, N-ethyl-N′-[(3-diethylamino)-propyl] carbodimide hydrochloride; FDA, U.S. Food and Drug Administration; GLP, good laboratory practice; GMP, good manufactur
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Evaluation of a biosensor assay to quantify polyclonal IgG in ovine serum used for the production of biotherapeutic antibody fragments
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 4, April 2006, Pages 842–847
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us