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Biohydrogen production from sucrose using base-enriched anaerobic mixed microflora

Paper ID Volume ID Publish Year Pages File Format Full-Text
36623 45138 2006 5 PDF Available
Title
Biohydrogen production from sucrose using base-enriched anaerobic mixed microflora
Abstract

A base-enriched anaerobic mixed microflora was used for hydrogen fermentation from sucrose in a laboratory scale model completely stirred tank bioreactor operating at 35 °C. The purpose of the study was to determine the microflora hydrogenic activity and its effects from the change of hydraulic retention time (HRT, 2–12 h). The experimental results indicate that base-enriched mixed microflora could be used as the seed for efficient hydrogen fermentation. The fermenter could operate stably for 250 days at a HRT of 12 h. Hydrogen gas content, hydrogen productivity and hydrogen production rate were HRT-dependent and their values ranged 38.7–45.9%, 0.9–3.5 mol H2/mol sucrose and 263–408 mmol H2/L day, respectively, with a HRT of 4 h having peak hydrogen production. The biomass activity was also HRT-dependent with each gram of biomass producing 65–145 mmol H2/day. The DGGE analysis shows that the microbial species shifted during the HRT-reduction operation but Clostridium ramosum was dominant. Those hydrogen productivity values were comparable to the hydrogen production using pure cultures, other mixed microflora or other reactor systems. The major liquid fermentation products were ethanol, acetic, propionic and butyric acids; their concentrations were also HRT-dependent. Strategies based on these results for optimal hydrogen production were proposed.

Keywords
Base-enrichment; Clostridium; Hydrogen production; Mixed microflora; Sucrose; Volatile fatty acid
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Biohydrogen production from sucrose using base-enriched anaerobic mixed microflora
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 4, April 2006, Pages 915–919
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us