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High cell density cultivation of recombinant E. coli with a pressurized culture

Paper ID Volume ID Publish Year Pages File Format Full-Text
36624 45138 2006 5 PDF Available
Title
High cell density cultivation of recombinant E. coli with a pressurized culture
Abstract

An air-pressurized culture was used to enhance the oxygen transfer rate in the high cell density cultivation (HCDC) of non-recombinant and recombinant (harbors pBR322trpAB) Escherichia coli K-12. Carbon dioxide generated by the cells under increased pressure was inhibitory and as a result, cellular growth stopped in the air-pressurized culture at a constant mass flow rate. Increasing the flow rate along with the pressure in the reactor enabled the cells to grow to 130 (non-recombinant) and 104 (recombinant) g/l due to the release of the CO2. In addition, the specific activity of tryptophan synthase was increased.This study showed that an air-pressurized culture is able to meet the high demand for oxygen in the HCDC of E. coli.

Keywords
Escherichia coli; Tryptophan synthase; Pressurized culture; CO2; HCDC
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 4, April 2006, Pages 920–924
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
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Any Questions? feel free to contact us