fulltext.study @t Gmail

Rapid non-invasive monitoring of baculovirus infection for insect larvae using green fluorescent protein reporter under early-to-late promoter and a GFP-specific optical probe

Paper ID Volume ID Publish Year Pages File Format Full-Text
36629 45138 2006 4 PDF Available
Title
Rapid non-invasive monitoring of baculovirus infection for insect larvae using green fluorescent protein reporter under early-to-late promoter and a GFP-specific optical probe
Abstract

We investigated monitoring for baculovirus infection of insect Trichoplusia ni larvae by combining the green fluorescent protein (GFP) reporter, the early-to-late (ETL) baculoviral promoter, and a GFP-specific optical probe. Because GFP is under the ETL promoter control, it facilitated rapid monitoring of recombinant baculovirus infection on insect larva system. Employment of GFP-specific optical probe also enabled non-invasive and accurate GFP monitoring with ∼10 h prior to invasive and GFP Western blot assay. This combination may assist in monitoring protein production runs, determining optimal infection and harvest timing, and in general, help to increase the yield of recombinant proteins expressed in larvae.

Keywords
Baculovirus infection; Insect larvae; Early-to-late promoter; Green fluorescent protein; GFP-specific optical probe; Non-invasive monitoring
First Page Preview
Rapid non-invasive monitoring of baculovirus infection for insect larvae using green fluorescent protein reporter under early-to-late promoter and a GFP-specific optical probe
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 4, April 2006, Pages 947–950
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us