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Production, purification and partial characterization of a novel protease from marine Engyodontium album BTMFS10 under solid state fermentation

Paper ID Volume ID Publish Year Pages File Format Full-Text
36631 45138 2006 6 PDF Available
Title
Production, purification and partial characterization of a novel protease from marine Engyodontium album BTMFS10 under solid state fermentation
Abstract

Engyodontium album isolated from marine sediment produced protease, which was active at pH 11. Process parameters influencing the production of alkaline protease by marine E. album was optimized. Particle size of <425 μm, 60% initial moisture content and incubation at 25 °C for 120 h were optimal for protease production under solid state fermentation (SSF) using wheat bran. The organism has two optimal pH (5 and 10) for maximal enzyme production. Sucrose as carbon source, ammonium hydrogen carbonate as additional inorganic nitrogen source and amino acid leucine enhanced enzyme production during SSF. The protease was purified and partially characterized. A 16-fold purified enzyme was obtained after ammonium sulphate precipitation and ion-exchange chromatography. Molecular weight of the purified enzyme protein was recorded approximately 38 kDa by SDS-PAGE. The enzyme showed maximum activity at pH 11 and 60 °C. Activity at high temperature and high alkaline pH suggests suitability of the enzyme for its application in detergent industry.

Keywords
Engyodontium album; Alkaline protease; Detergent enzyme; Solid state fermentation; Enzyme purification
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Production, purification and partial characterization of a novel protease from marine Engyodontium album BTMFS10 under solid state fermentation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 41, Issue 4, April 2006, Pages 956–961
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
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