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Primary recovery of lipase derived from Burkholderia cenocepacia strain ST8 and recycling of phase components in an aqueous two-phase system

Paper ID Volume ID Publish Year Pages File Format Full-Text
3664 181 2012 7 PDF Available
Title
Primary recovery of lipase derived from Burkholderia cenocepacia strain ST8 and recycling of phase components in an aqueous two-phase system
Abstract

The recovery of Burkholderia cenocepacia (B. cenocepacia) strain ST8 lipase has been investigated using a recycling aqueous two-phase system (ATPS) consisting of the thermoseparating random copolymer ethylene oxide–propylene oxide (EOPO) and potassium phosphate. In the primary ATPS, lipase was satisfactorily partitioned to the polymer-rich top phase in the system composed of EOPO 3900 with a tie-line length (TLL) of 48.5% (w/w), a volume ratio (VR) of 2.3 and a crude load of 20% (w/w) at pH 7. In the secondary system, the polymer-rich top phase from the primary ATPS was isolated and subjected to thermo-induced phase separation. After thermoseparating, a concentrated solution of EOPO copolymer was formed in the bottom phase, and a water solution containing lipase was formed in the top phase of this secondary two-phase system. Recycling of the components was accomplished by mixing the concentrated polymer recovered from the secondary system and the phosphate solution recovered from the primary system. The average purification factor of lipase and the yield obtained from the four successive purifications were 14 and 99%, respectively. There was no significant difference in the purification factor, yield or partitioning efficiency of purified lipase obtained using fresh or recycled chemicals in the ATPS.

► Recovery of lipase using an ATPS composed of EOPO/K2H2PO4 with recycling of the phase components. ► EOPO 3900 with a TLL of 48.5% (w/w) was selected as the optimum system. ► Optimum recovery was obtained at a VR of 2.3 and a crude load of 20% (w/w) at pH 7. ► The purification factor and yield using recycled components was 14 and 99%, respectively.

Keywords
Lipase; Aqueous two-phase; Purification; Protein recovery; Bioseparations; Enzymes
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Primary recovery of lipase derived from Burkholderia cenocepacia strain ST8 and recycling of phase components in an aqueous two-phase system
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 60, 15 January 2012, Pages 74–80
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us