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Optimization of medium constituents for a novel lipopeptide production by Bacillus subtilis MO-01 by a response surface method

Paper ID Volume ID Publish Year Pages File Format Full-Text
36645 45139 2005 6 PDF Available
Title
Optimization of medium constituents for a novel lipopeptide production by Bacillus subtilis MO-01 by a response surface method
Abstract

The production of a novel lipopeptide by Bacillus subtilis MO-01 was investigated in shaker flask fermentation. Response surface methodology (RSM) was applied to optimize the medium ingredients. A five-level four-factor central composite design was employed to determine the maximum lipopeptide yield at optimum levels for sucrose, ammonium chloride, ferrous sulphate and zinc sulphate. Sucrose, ferrous sulphate and zinc sulphate showed a significant linear main effect, while ammonium chloride had no significant linear effect. The production was also significantly affected by sucrose-ferrous sulphate and sucrose-zinc sulphate, as well as by the negative quadric effect of the above four variables. Optimum fermentation parameters were 22.431 g/l of sucrose, 2.781 g/l of ammonium chloride and 6.7879 μM of 0.0377 mM of zinc sulphate. The prediction lipopeptide yield was 1.712 g/l. The actual experimental results were in agreement with the prediction.

Keywords
Lipopeptide; Bacillus subtilis; Media optimization; Response surface method
First Page Preview
Optimization of medium constituents for a novel lipopeptide production by Bacillus subtilis MO-01 by a response surface method
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 40, Issue 10, October 2005, Pages 3196–3201
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering