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Restricted electron flux increases coenzyme Q10 production in Agrobacterium tumefaciens ATCC4452

Paper ID Volume ID Publish Year Pages File Format Full-Text
36650 45139 2005 5 PDF Available
Title
Restricted electron flux increases coenzyme Q10 production in Agrobacterium tumefaciens ATCC4452
Abstract

Coenzyme Q10 (CoQ10) is an electron carrier in the respiration chain with antioxidant activity. The effects of aeration and agitation rates, dissolved oxygen levels, an electron flux inhibitor (azide), a proton gradient releaser (2,4-dinitrophenol (DNP)), and an oxidative stressor (hydrogen peroxide (H2O2)) on the intracellular CoQ10 contents in Agrobacterium tumefaciens ATCC4452 were investigated. With a decrease of dissolved oxygen level from 20 to 5%, the intracellular CoQ10 content increased about four-fold, yielding 2 mg/g dry cell weight at 5% dissolved oxygen level. Azide significantly increased the intracellular CoQ10 content, with the highest value of 5.3 mg/g dry cell weight in the presence of 0.45 mM of sodium azide. However, DNP (up to 200 μM) and H2O2 (up to 10 μM) did not affect the intracellular CoQ10 content, indicating proton gradient release, and oxidative stress do not affect the synthesis of CoQ10. These results show that restricted electron flux by limited oxygen supply and the addition of azide increases the intracellular CoQ10 content, suggesting a feedback regulation of CoQ10 biosynthesis by its physiological function of electron carrier in the respiration chain.

Keywords
Coenzyme Q10; Agrobacterium tumefaciens; Dissolved oxygen; Sodium azide; DNP; Hydrogen peroxide
First Page Preview
Restricted electron flux increases coenzyme Q10 production in Agrobacterium tumefaciens ATCC4452
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 40, Issue 10, October 2005, Pages 3225–3229
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering