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Isolation and partial characterization of antifungal protein from Bacillus polymyxa strain VLB16

Paper ID Volume ID Publish Year Pages File Format Full-Text
36652 45139 2005 8 PDF Available
Title
Isolation and partial characterization of antifungal protein from Bacillus polymyxa strain VLB16
Abstract

An antifungal protein produced by Bacillus polymyxa strain VLB16 has been purified to homogeneity by ammonium sulfate precipitation and Sephadex-G-200 column chromatography. The purified protein inhibited the growth of Pyricularia grisea and Rhizoctonia solani, the causative agents of rice blast and sheath blight diseases, respectively. Microscopy studies revealed that the antifungal protein caused severe alterations in cell morphogenesis that gave rise to swollen and rounded hyphae. The protein was a monomer with molecular weight of 37 kDa in sodium dodecyl sulfate-poly acrylamide gel electrophoresis and gave a single symmetrical peak in gel permeation chromatography. The presence of predominant random coil structure and other conformations such as β-sheet and β-turn was noticed from Fourier transform infra red spectroscopy. The protein was heat stable and remained active after sterilization at 121 °C for 15 min. The antifungal protein was resistant to hydrolysis by pronase and also to many protein-denaturing detergents like sodium dodecyl sulfate (SDS), hexa decyl trimethyl ammonium bromide (HDTMA) and Tween-20. The extraordinary thermo-stability of the protein can be of good prospect to be used as a new tool for biocontrol.

Keywords
AFP, antifungal protein; HDTMA, hexa decyl trimethyl ammonium bromide; LBA, luria-bertaini agar; MIC, minimum inhibitory concentration; PDA, potato dextrose agar; PDB, potato dextrose broth; VLB, VilliambakkamAntifungal protein; Bacillus polymyxa; Purific
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Isolation and partial characterization of antifungal protein from Bacillus polymyxa strain VLB16
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 40, Issue 10, October 2005, Pages 3236–3243
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us