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Purification, characterization and some studies on secondary structure of tannase from Aspergillus awamori nakazawa

Paper ID Volume ID Publish Year Pages File Format Full-Text
36654 45139 2005 4 PDF Available
Title
Purification, characterization and some studies on secondary structure of tannase from Aspergillus awamori nakazawa
Abstract

Tannase (tannin acyl hydrolase EC 3.1.1.20) produced by Aspergillus awamori nakazawa was purified and characterized. Optimal conditions of production were determined using varying substrate combinations and studying fermentation on various media combinations. Fermentation was carried out for 46 h for optimum enzyme production. Enzyme samples were obtained from the broth after fermentation by acetone precipitation of the supernatant followed by gel filtration chromatography. The properties of the enzyme were investigated. The optimum conditions of temperature and pH were investigated and the effects of urea, surfactant and chelator were studied. Tannase from this new isolate exhibited optimum activity at 35 °C and at a pH of 5.0. Urea concentrations higher than 3 M were inhibitory. Increasing concentrations of sodium lauryl sulphate also led to decrease in activity. Two percent SLS was inhibitory. Increasing concentrations of EDTA had an inhibitory effect on tannase. Tannase was found to be a glycoprotein. Circular dichroism analysis of purified fractions of tannase indicates that the β-sheet structure in tannase is predominant indicating its globular nature.

Keywords
Fungal tannase; Tannase substrate; Tannase assay; Glycoprotein; GFC; HPLC; CD
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Purification, characterization and some studies on secondary structure of tannase from Aspergillus awamori nakazawa
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 40, Issue 10, October 2005, Pages 3251–3254
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us