fulltext.study @t Gmail

Continuous cyclodextrin glucanotransferase production by free and immobilized cells of Bacillus circulans ATCC 21783 in bioreactors

Paper ID Volume ID Publish Year Pages File Format Full-Text
36661 45139 2005 6 PDF Available
Title
Continuous cyclodextrin glucanotransferase production by free and immobilized cells of Bacillus circulans ATCC 21783 in bioreactors
Abstract

Four systems for a continuous cyclodextrin glucanotransferase (CGTase) production by Bacillus circulans ATCC 21783 were studied: free cells in a stirred reactor; free cells in a bubble column reactor; agar-entrapped and membrane-immobilized cells in a bubble column reactor. The performance of the process with free cells led to a higher enzyme level, specific CGTase activity and enzyme productivity compared to systems using immobilized cells. However, the residual enzyme activity (after approximately 300 h process) was from 96 to 97% for immobilized cells and from 78 to 86% for free cells. The four used systems provided a significantly higher CGTase yield (from 3.4-fold to 4.7-fold) and productivity (from 1.4-fold to 2.9-fold) in comparison to known strains of Bacillus applied for the same aim. The concomitant high specific CGTase activity indicated that the established cultural conditions provided the dominant synthesis of the active protein.

Keywords
Bacillus circulans; Cyclodextrin glucanotransferase; Cyclodextrins; Continuous enzyme production; Free and immobilized cells; Bioreactors
First Page Preview
Continuous cyclodextrin glucanotransferase production by free and immobilized cells of Bacillus circulans ATCC 21783 in bioreactors
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 40, Issue 10, October 2005, Pages 3290–3295
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us