fulltext.study @t Gmail

Bioconversion of methyl-testosterone in a biphasic system

Paper ID Volume ID Publish Year Pages File Format Full-Text
36664 45139 2005 5 PDF Available
Title
Bioconversion of methyl-testosterone in a biphasic system
Abstract

The bioconversion of methyl-testosterone to methandienone in a biphasic system was studied using freeze-dried, thawed and growing Arthrobacter simplex AS 1.94* cells as biocatalyst. The biphasic system consists of an organic phase [30% (v/v)] with steroid 10 g/L and an aqueous phase [70% (v/v)] containing the cells (5 g.d.c.w/L). Carbon tetrachloride and Tween-80 were used as organic solvent and surfactant, respectively, and menadione was added as an external electron acceptor. The factors affecting the conversion rate in the two-phase system were investigated. The results showed that menadione was necessary in this system. Small amounts of product added to the reaction system can increase the dehydrogenation rate. Higher activities were obtained with thawed cells as compared to the freeze-dried and growing cells. Under the optimal operation conditions, more than 95% (w/w) of added methyl-testosterone could be converted to methandienone within 48 h. Also, an easy way to recover the product in a high purity is proposed.

Keywords
Methyl-testosterone; Methandienone; Bioconversion; Arthrobacter simplex; Δ1,2-Dehydrogenation; A biphasic system
First Page Preview
Bioconversion of methyl-testosterone in a biphasic system
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 40, Issue 10, October 2005, Pages 3309–3313
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us