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Concomitant production and downstream processing of alkaline protease and biosurfactant from Bacillus licheniformis RG1: Bioformulation as detergent additive

Paper ID Volume ID Publish Year Pages File Format Full-Text
36670 45139 2005 8 PDF Available
Title
Concomitant production and downstream processing of alkaline protease and biosurfactant from Bacillus licheniformis RG1: Bioformulation as detergent additive
Abstract

Concomitant production of protease and biosurfactant was optimized using agro-products, namely cornstarch and soy flour as carbon and nitrogen sources, respectively, by response surface methodology. An overall 2.3-fold increase for both protease (2954 U/ml) and biosurfactant (41%) was achieved. Ultrafilteration using a 100 kDa membrane allowed a cost-effective purification of both protease and biosurfactant where the surfactant traps the protease on the membrane thereby detaining both in the retentate. These products were found to have a shelf life of 1 year in the dry form prepared by mixing sodium sulphate in the retentate. Thus, concomitant production of protease and surfactant, their easy formulation makes them attractive detergent additives.

Keywords
Bacillus licheniformis; Bioformulation; Biosurfactant; Detergent additive; Protease; Response surface methodology
First Page Preview
Concomitant production and downstream processing of alkaline protease and biosurfactant from Bacillus licheniformis RG1: Bioformulation as detergent additive
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 40, Issue 10, October 2005, Pages 3352–3359
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering