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An artificial neural network analysis of porcine pancreas lipase catalysed esterification of anthranilic acid with methanol

Paper ID Volume ID Publish Year Pages File Format Full-Text
36673 45139 2005 5 PDF Available
Title
An artificial neural network analysis of porcine pancreas lipase catalysed esterification of anthranilic acid with methanol
Abstract

Artificial neural network (ANN) analysis of porcine pancreas lipase (PPL) catalysed esterification of anthranilic acid with methanol was carried out. Methanol concentration, enzyme concentration, period of incubation, buffer volume and log P-values were the five important parameters employed. ANN analysis showed good correspondence between experimental and predicted values. The surface plots showed that methanol bound to PPL in preference to anthranilic acid. Compared to anthranilic acid (0.1 M) methanol was bound to PPL preferentially up to 0.5 M. Increase in enzyme concentration was found to enhance the extent of esterification in the concentration range 0.5–4.5 mg/mL of PPL. A similar increase in esterification was also observed in the presence of 0.05–0.35 mL of 0.1 M phosphate buffer at pH 7.0. The positive effect of the buffer indicated that imparting ‘pH memory’ to the enzyme enhanced the extent of esterification. Esterification increased also when it was carried out in non-polar solvents like n-heptane which has a log P-value of 4.0 compared to benzene (log P: 2.0) indicating that non-polar solvents were very effective in stabilising PPL especially when they contained a small amount of 0.1 M phosphate buffer at pH 7.0.

Keywords
Artificial neural network; Porcine pancreas lipase; Esterification; Methyl anthranilate; Inhibition; Buffer volume; Log P-value; pH memory
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An artificial neural network analysis of porcine pancreas lipase catalysed esterification of anthranilic acid with methanol
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 40, Issue 10, October 2005, Pages 3372–3376
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us