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The use of proteases to remove protein-based residues from solid surfaces

Paper ID Volume ID Publish Year Pages File Format Full-Text
36674 45139 2005 6 PDF Available
Title
The use of proteases to remove protein-based residues from solid surfaces
Abstract

The efficiency of the commercial proteolytic preparation ‘Alcalase’ in removing model proteins (casein, BSA and haemoglobin) experimentally burned onto a model solid surface (glass) was investigated. The cleaning efficiency was monitored qualitatively by direct observation and quantitatively by monitoring polypeptide/peptide/amino acid accumulation in the wash and by three-dimensional atomic force microscopy. The results indicate that treatment with enzyme at industrially realistic inclusion levels removes a very significant proportion of protein foulant and that post-cleaning residual enzyme activity on the glass surface can be easily removed or inactivated. Results are discussed in the context of their potential application in the bioprocess sector.

Keywords
Alcalase; Protease; Enzyme-cleaning; Fouling; Glass
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The use of proteases to remove protein-based residues from solid surfaces
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 40, Issue 10, October 2005, Pages 3377–3382
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us