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Medium engineering to enhance mushroom tyrosinase stability

Paper ID Volume ID Publish Year Pages File Format Full-Text
3668 181 2012 7 PDF Available
Title
Medium engineering to enhance mushroom tyrosinase stability
Abstract

Tyrosinase could have many applications including biotransformation, bioremediation, and in biosensors. However, its application, especially at industrial scale, is seriously limited by its rapid inactivation. To increase mushroom tyrosinase (MT) stability, the enzyme was entrapped in different matrixes. Then, the structural and operational stability of the entrapped mushroom tyrosinase (EMT) were examined in one-phase-binary solutions (OPBS) obtained by mixing different amounts (0–100%) of water-miscible solvents such as acetonitrile and 2-propanol with PBS. Experiments showed MT lost all of its activity upon entrapment in the classical sol–gels and hybrid silica sol–gels, but could retain up to 25% of its activity in cross-linked polyacrylamide (CLP). These studies revealed while EMT in CLP was losing about 0.06% of its structural stability per hour in PBS, OPBS containing 2-propanol (50 or 75%) did not affect the EMT structural stability and increased its cresolase activity up to 1.5 fold during 50 days storage at 4 °C. The aquaphilicity of CLP helps EMT to function normally in OPBS and using 2-propanol (50% or more) in such media not only stopped EMT loss from the sieve structure of the CLP, but it was also beneficial to the more hydrophobic substrates. Using tyrosine as a substrate in OPBS containing 2-propanol (75%), l-DOPA production reached 4.21 ± 0.23 μM/min−1.

► Development of an entrapping method for mushroom tyrosinase. ► Studying the combination of one-phase-binary solution and cross-linked polyacrylamide for entrapping enzymes. ► Changes in the functional and structural properties of mushroom tyrosinase under the conditions selected.

Keywords
Ac, acrylamide; APS, ammonium persulfate; bAc, bis-acrylamide; CLP, cross-linked polyacrylamide; DC, denaturation capacity; EMT, entrapped mushroom tyrosinase; MePAPh, 4-[(4-methylphenyl)azo]-phenol; MeTPS, methyltripropoxysilane; MT, mushroom tyrosinase;
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 60, 15 January 2012, Pages 99–105
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us