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Microbubble fermentation of recombinant Pichia pastoris for human serum albumin production

Paper ID Volume ID Publish Year Pages File Format Full-Text
36687 45142 2005 6 PDF Available
Title
Microbubble fermentation of recombinant Pichia pastoris for human serum albumin production
Abstract

The high cell density fermentation of recombinant Pichia pastoris for human serum albumin (HSA) production is a high oxygen demand process. The oxygen demand is usually met by increased agitation rate and use of oxygen-enriched air. However, fermentation using a microbubble dispersion (MBD) can supply adequate oxygen to the microorganisms at relatively low agitation rates because of improved oxygen mass-transfer of the microbubbles used for the sparging. Conventional air-sparged fermentations were conducted for the production of HSA using P. pastoris at agitation rates of 350, 500, 750 rpm. These experiments were compared to MBD-sparged fermentation at 350 and 500 rpm agitation rates. After 97 h of air-sparged fermentation, cell masses were 27.9, 48.9, and 138.2 g/l at agitation rates of 350, 500, and 750 rpm, respectively. For MBD-sparged fermentation, cell mass concentrations after 97 h were 129.9 and 137.7 g/l at 350 and 500 rpm, respectively. Protein concentrations were 36.3 and 67.8 mg/l for air-sparged at 350 and 500 rpm, while 267.3 and 301.2 mg/l for MBD-sparged experiments at 350 and 500 rpm, respectively. The volumetric oxygen mass-transfer coefficient, kLa for the MBD system at 500 rpm was 268.6 h−1 compared to 76.1 h−1 for the conventional sparger at 500 rpm. There was significant improvement in oxygen transfer to the microorganism relative to the conventional sparger.

Keywords
Microbubble; Oxygen transfer; Pichia pastoris
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Microbubble fermentation of recombinant Pichia pastoris for human serum albumin production
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 40, Issue 6, May 2005, Pages 2073–2078
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us