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By-product formation in the d-sorbitol to l-sorbose biotransformation by Gluconobacter suboxydans ATCC 621 in batch and continuous cultures

Paper ID Volume ID Publish Year Pages File Format Full-Text
36692 45142 2005 10 PDF Available
Title
By-product formation in the d-sorbitol to l-sorbose biotransformation by Gluconobacter suboxydans ATCC 621 in batch and continuous cultures
Abstract

Gluconobacter suboxydans was used in the biotransformation of d-sorbitol to l-sorbose, a key step in the industrial manufacture of Vitamin C (ascorbic acid). However, despite evidence of the existence of a range of by-products in the bioprocess, relatively little scientific investigation has focused on the factors influencing by-product formation in this complex bioprocess. The aim of the present study was therefore to investigate systematically the extent of by-product formation in this economically important biotransformation process using batch and continuous cultivation methods and up to date analytical techniques and, thus, to arrive at a realistic assessment of the yield of sorbose via this route. The results presented here show that exogenous by-product addition to shake flask and batch cultures detrimentally affects sorbose production and yield, but can have some positive effects in continuous culture. Some inhibition of fructose accumulation by mannitol-1-phosphate addition was noted in shake flask cultures.

Keywords
Biotransformations; Fermentation; Submerged culture; Fructose; Gluconobacter; Sorbose
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By-product formation in the d-sorbitol to l-sorbose biotransformation by Gluconobacter suboxydans ATCC 621 in batch and continuous cultures
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 40, Issue 6, May 2005, Pages 2113–2122
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us