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Utilization of bentonite as a support material for immobilization of Candida rugosa lipase

Paper ID Volume ID Publish Year Pages File Format Full-Text
36697 45142 2005 5 PDF Available
Title
Utilization of bentonite as a support material for immobilization of Candida rugosa lipase
Abstract

Bentonite is an inexpensive matrix for enzyme immobilization and has been frequently utilized for this purpose. In addition to its low cost, bentonite has several advantages for use as a support, including its lack of toxicity and chemical reactivity, allowing easy fixation of enzymes. In this article, Candida rugosa lipase was non-covalently immobilized on bentonite. The properties of immobilized enzyme were defined. Optimum pH and water content were determined as 7.5 and 100 μL, respectively. The patterns of heat stability indicated that the immobilization process tends to stabilize the enzyme. Half-lives of the immobilized enzyme at 50, 60, and 70 °C were 45, 15, and 4 min, respectively, whereas for the soluble free lipase it was 17 min at 50 °C. Kinetics were tested at 37 °C following the hydrolysis of olive oil and obeys the Lineweaver–Burk type of rate equation. The Km was 4 × 10−2 mM and the Vmax was 15 mmol h−1 mg−1.

Keywords
Candida rugosa lipase; Enzyme immobilization; Bentonite; Enzymes; Organic solvents; Kinetic properties
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Utilization of bentonite as a support material for immobilization of Candida rugosa lipase
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 40, Issue 6, May 2005, Pages 2155–2159
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us