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Preparation of enantiopure (S)-ketoprofen by immobilized Candidarugosa lipase in packed bed reactor

Paper ID Volume ID Publish Year Pages File Format Full-Text
36698 45142 2005 6 PDF Available
Title
Preparation of enantiopure (S)-ketoprofen by immobilized Candidarugosa lipase in packed bed reactor
Abstract

Various materials have been used as supports for the immobilization of a Candidarugosa lipase (Lipase OF). The enzyme adsorbed on silica gel showed the maximum activity recovery (37%), and was thus used to construct a packed bed reactor for the continuous production of (S)-ketoprofen. The performance of the bioreactor containing 4.0 g immobilized biocatalyst was evaluated as a function of parameters such as flow rate, the ratio of height to diameter (H/D) and substrate concentration. The space velocity (SV) of the reactor showed a linear increase when the flow rate was increased in the range of 5.9 (SV = 0.9 h−1)–25 ml h−1 (SV = 3.9 h−1) and leveled out at values for flow rate between 25 (SV = 3.9 h−1) and 64 ml h−1 (SV = 10.2 h−1). The initial rate remained the same when the H/D was varied from 2.9 to 38.6. The initial rate rose in a linear fashion as the substrate concentration was increased from 5 to 20 mg ml−1 and remained almost at the same level when the substrate concentration was between 20 and 50 mg ml−1. Using a packed bed bioreactor, optically pure (S)-ketoprofen (>99% ee (enantiomeric excess)) was produced with a conversion of 30% and productivity of 1.5 mg g−1 biocatalyst h−1.

Keywords
Ketoprofen; Kinetic resolution; Enantioselective hydrolysis; Candidarugosa lipase; Immobilization; Packed bed reactor
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Preparation of enantiopure (S)-ketoprofen by immobilized Candidarugosa lipase in packed bed reactor
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 40, Issue 6, May 2005, Pages 2161–2166
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us