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Production and optimization of process parameters for alkaline protease production by a newly isolated Bacillus sp. under solid state fermentation

Paper ID Volume ID Publish Year Pages File Format Full-Text
36718 45146 2004 6 PDF Available
Title
Production and optimization of process parameters for alkaline protease production by a newly isolated Bacillus sp. under solid state fermentation
Abstract

Production of alkaline protease employing the laboratory isolate, Bacillus sp. under solid state fermentation (SSF) was optimized. The effect of wheat bran and lentil husk was examined. Wheat bran showed highest enzyme production. The appropriate incubation time, inoculum size, moisture level and buffer solution level were determined. Maximum yields of 429.041 and 168.640 U g−1 were achieved by employing wheat bran and lentil husk as substrates in 0.1 M carbonate/bicarbonate buffer at pH 10 with 30 and 40% initial moisture level at 24 h. Inoculum size and buffer solution level were found to be 20 and 25% and 0.5:1 for wheat bran and lentil husk, respectively.

Keywords
Solid state fermentation; Bacillus; Alkaline protease
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Production and optimization of process parameters for alkaline protease production by a newly isolated Bacillus sp. under solid state fermentation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 39, Issue 12, 29 October 2004, Pages 1893–1898
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
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Price was $35.95
You save - $31
Price after discount Only $4.95
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