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Oxygen limitation improves glycerol production by Candida krusei in a bioreactor

Paper ID Volume ID Publish Year Pages File Format Full-Text
36719 45146 2004 4 PDF Available
Title
Oxygen limitation improves glycerol production by Candida krusei in a bioreactor
Abstract

An oxygen limitation strategy based on dynamic enzyme activity was applied to improve glycerol accumulation and decrease the residual sugar level in a fermentation of Candida krusei in a bioreactor. By applying oxygen limitation at 88 h when the activities of two glycerol synthetic enzymes cytosolic glycerol-3-phosphate dehydrogenase (ctGPD) and glycerol-3-phosphatase (GPP) were low and the activity of mitochondrial glycerol-3-phosphate dehydrogenase (mtGPD) which catalyzes the glycerol dissimilation was high, the glycerol dissimilation was efficiently reduced. The final glycerol concentration reached 51.8 g l−1 at 96 h and 54.9 g l−1 at 116 h, which was 18 and 60% higher than the control (without oxygen limitation), respectively. The residual sugar was consumed completely while it was 11.2 g l−1 at the end of fermentation in the control. Under oxygen limitation, ethanol production was detected at a final concentration of 3.6 g l−1. This work suggests a metabolic flux shift by oxygen limitation in the bioreactor.

Keywords
Candida krusei; Oxygen limitation; Glycerol fermentation; Cytosolic glycerol-3-phosphate dehydrogenase (ctGPD); Glycerol-3-phosphatase (GPP); Mitochondrial glycerol-3-phosphate dehydrogenase (mtGPD)
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Oxygen limitation improves glycerol production by Candida krusei in a bioreactor
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 39, Issue 12, 29 October 2004, Pages 1899–1902
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
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Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
Online Support
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