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Characteristics of invertase partitioned in poly(ethylene glycol)/magnesium sulfate aqueous two-phase system

Paper ID Volume ID Publish Year Pages File Format Full-Text
3674 181 2012 9 PDF Available
Title
Characteristics of invertase partitioned in poly(ethylene glycol)/magnesium sulfate aqueous two-phase system
Abstract

The goal of this study was to determine some characteristics of Baker's yeast invertase partitioned with poly(ethylene glycol)/MgSO4 aqueous two-phase system (ATPS). Under optimized conditions [PEG-3000 (15%, w/w) and MgSO4 (23%, w/w) with 5% (w/w) MnCl2 at pH 5.0] yeast invertase was partitioned by using an ATPS with purification factor of 6.2-fold and activity recovery of 217.7%, respectively. The yeast invertase was characterised with respect to its activity and stability at various pH and temperature ranges. Optimum pH and temperature were determined at pH 5.5 and 60 °C, respectively. The enzyme was very stable in the range of pH 4.0–7.0 and more than 95% of its initial activity was recovered. The yeast invertase was also stable at the temperature range of 4–50 °C and retained nearly about 98% of its initial activity at 50 °C. Kinetic parameters, Km and Vmax using sucrose as substrate were measured as 24.1 mM and 35.5 U, respectively. MnSO4 and MnCl2 efficiently enhanced the activity and also showed an activator effect for invertase. Relative activities were found as 151% and 156% for MnSO4 and MnCl2, respectively. The biochemical properties of the yeast invertase partitioned in an ATPS make the enzyme good candidates for several industrial applications.

► Invertases have many biotechnological applications especially in food processing. ► ATPSs are powerful and attractive for separating of various biomolecules. ► Characterisation of invertase partitioned with an ATPS was searched.

Keywords
Baker's yeast; Invertase; Aqueous-two phase system; Bioseparation; Partitioning; Characterisation
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Characteristics of invertase partitioned in poly(ethylene glycol)/magnesium sulfate aqueous two-phase system
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 60, 15 January 2012, Pages 142–150
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us