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Extraction and purification of phycocyanin from Calothrix sp.

Paper ID Volume ID Publish Year Pages File Format Full-Text
36740 45146 2004 6 PDF Available
Title
Extraction and purification of phycocyanin from Calothrix sp.
Abstract

The extraction and purification of phycocyanin from Calothrix sp., cyanobacteria isolated from rice fields in Cuernavaca, Morelos, Mexico is described. Phycocyanin was extracted with 2 mg of lysozyme/g wet biomass, and purified by anion chromatography using Q-Sepharose fast-flow (Pharmacia®,  cm) column and hydrophobic interaction chromatography with methyl macro-prep (Bio-Rad®,  cm) column. The purified protein showed a pI of 5.2 and has two subunits with apparent molecular mass of 21–17 kDa each. The estimated molecular mass of native purified phycocyanin was 114 kDa, suggesting a stereochemistry of (αβ)3.

Keywords
Calothrix sp.; Cyanobacteria; Lysozyme; Phycocyanin; Extraction; Purification
First Page Preview
Extraction and purification of phycocyanin from Calothrix sp.
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 39, Issue 12, 29 October 2004, Pages 2047–2052
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering