Extraction and purification of phycocyanin from Calothrix sp.
The extraction and purification of phycocyanin from Calothrix sp., cyanobacteria isolated from rice fields in Cuernavaca, Morelos, Mexico is described. Phycocyanin was extracted with 2 mg of lysozyme/g wet biomass, and purified by anion chromatography using Q-Sepharose fast-flow (Pharmacia®, cm) column and hydrophobic interaction chromatography with methyl macro-prep (Bio-Rad®, cm) column. The purified protein showed a pI of 5.2 and has two subunits with apparent molecular mass of 21–17 kDa each. The estimated molecular mass of native purified phycocyanin was 114 kDa, suggesting a stereochemistry of (αβ)3.
Journal: Process Biochemistry - Volume 39, Issue 12, 29 October 2004, Pages 2047–2052