fulltext.study @t Gmail

Optimization of cultural conditions for enhancing the expression of human interferon-α2a by recombinant yeast

Paper ID Volume ID Publish Year Pages File Format Full-Text
36743 45146 2004 5 PDF Available
Title
Optimization of cultural conditions for enhancing the expression of human interferon-α2a by recombinant yeast
Abstract

When adenine (Ade) was added to basal culture medium at 12 h after inoculation, antivirus activity and specific activity of interferon-α2a (IFN-α2a) were increased. Expression of IFN-α2a was realized when a low residual glucose concentration was maintained during the mid- and late-phase of cultivation. In addition, biological activity and specific activity of IFN-α2a were increased by more than 100% if the ratio of glucose and sucrose in the basal medium was 1:0.1. The addition of glutamate also led to the intensive enhancement of the expression level of IFN-α2a. The initial pH of the basal medium proved to be crucial to the expression level. When the above optimal cultivation condition obtained in the shake flask was applied to the fed-batch culture using a 2.6 l jar fermenter, human IFN-α2a biological activity reached 1.3×107 IU/ml, which was four times that of the control.

Keywords
Interferon-α2a; Optimization; Fed-batch culture; Recombinant yeast: Saccharomyces cerevisiae
First Page Preview
Optimization of cultural conditions for enhancing the expression of human interferon-α2a by recombinant yeast
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 39, Issue 12, 29 October 2004, Pages 2069–2073
Authors
, , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us