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Effect of water activity on invertase production in solid state fermentation by improved diploid strains of Aspergillus niger

Paper ID Volume ID Publish Year Pages File Format Full-Text
36745 45146 2004 6 PDF Available
Title
Effect of water activity on invertase production in solid state fermentation by improved diploid strains of Aspergillus niger
Abstract

Invertase production under solid state fermentation (SSF) was determined using two overproducing mutants (Aw96-3 and Aw96-4) isolated previously from the wild type strain Aspergillus niger C28B25, as well as one diploid (DAR1) and two autodiploid strains (AD96-3 and AD96-4) constructed by parasexual crossings among these mutants. Using polyurethane foam (PUF) as an inert carrier, two initial water activity (Aw) values were evaluated (0.99 and 0.96). At Aw=0.99, maximal activity was reached by diploid AD96-4 (48.91 IU/ml) representing 30- and 13-fold increases with respect to maximal values achieved by the wild type and the haploid parental mutant (Aw96-4), respectively. Similar levels were achieved by this strain at Aw=0.96. However, diploid DAR1 only produced high levels of invertase at Aw=0.96 (43.90 IU/ml), whereas strain AD96-3 reached its highest production (31.10 IU/ml) at Aw=0.99. Both productivity and yields were also analysed for every strain at each Aw value.

Keywords
Aspergillus niger; Invertase; Overproduction; Parasexual cycle; Solid fermentation; Water activity; Enzymatic yields
First Page Preview
Effect of water activity on invertase production in solid state fermentation by improved diploid strains of Aspergillus niger
Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 39, Issue 12, 29 October 2004, Pages 2085–2090
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering