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Lipolytic activity in submerged cultures of Issatchenkia orientalis

Paper ID Volume ID Publish Year Pages File Format Full-Text
36748 45146 2004 6 PDF Available
Title
Lipolytic activity in submerged cultures of Issatchenkia orientalis
Abstract

The ability of the yeast Issatchenkia orientalis CECT 10688 to secrete lipolytic activity in submerged culture was investigated. The yeast was grown in a complex medium supplemented with a fixed concentration of several lipidic compounds (triglycerides, fatty acids). Maximum enzyme activity around 70–80 U cm−3 was produced in cultures supplemented with tributyrin. An optimum tributyrin concentration of 10 g dm−3 was selected. Several surfactants were added to the cultures, but no significant increase in activity was detected. Finally, the effect of the type of carbon source on lipolytic enzyme production was studied. The best results were obtained with glucose and fructose (60–80 U cm−3), while rather low enzyme activity was found in cultures grown on lactose and maltose (about 20 U cm−3).

Keywords
Lipase; Enzyme production; Surfactants; Fatty acids; Triglycerides; Carbon source
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 39, Issue 12, 29 October 2004, Pages 2109–2114
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Don't Miss Today's Special Offer
Price was $35.95
You save - $31
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