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Analysis of the expression of the Trichoderma harzianum ech42 gene in two isogenic clones of Escherichia coli by surface response methodology

Paper ID Volume ID Publish Year Pages File Format Full-Text
36757 45146 2004 6 PDF Available
Title
Analysis of the expression of the Trichoderma harzianum ech42 gene in two isogenic clones of Escherichia coli by surface response methodology
Abstract

The endochitinase gene ech42 from Trichoderma harzianum was cloned and expressed in Escherichia coli BL21(D3)pLysS using a pET-19b expression system. The effect of temperature, cell concentrations and inductor concentration on protein expression were evaluated by response surface methodology. Applying a second-order model to describe the amount of endochitinase produced by two selected clones, demonstrated that different optimal conditions of expression were found among clones, indicating that the best environmental conditions for expression among the several isogenic clones differed.

Keywords
Endochitinase; Trichoderma harzianum; Surface response methodology; Escherichia coli
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Analysis of the expression of the Trichoderma harzianum ech42 gene in two isogenic clones of Escherichia coli by surface response methodology
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 39, Issue 12, 29 October 2004, Pages 2173–2178
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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