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Effect of gamma-irradiation on guanyl specific ribonuclease from Trichoderma harzianum

Paper ID Volume ID Publish Year Pages File Format Full-Text
36769 45146 2004 4 PDF Available
Title
Effect of gamma-irradiation on guanyl specific ribonuclease from Trichoderma harzianum
Abstract

Sensitivity of aqueous solutions of fungal guanyl specific RNase Th to irradiation with different doses of γ-rays was studied. Enzyme activity, aggregation, amino acid composition, UV-absorption and difference spectra of native and irradiated enzyme were investigated. Inactivation of RNase Th after irradiation occurred with all doses used and alterations in the spectral properties of the enzyme were observed. The reasons of these deviations most likely are changes in the conformation as a result of breaking of hydrogen and disulfide bonds and destroying of one molecule of tyrosine. The data received for the sensitivity of this enzyme to gamma-irradiation could contribute to more detail characterization of this family of proteins.

Keywords
Gamma-irradiation; RNase Th; UV-spectra; Amino acid composition
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Effect of gamma-irradiation on guanyl specific ribonuclease from Trichoderma harzianum
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 39, Issue 12, 29 October 2004, Pages 2257–2260
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
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Price was $35.95
You save - $31
Price after discount Only $4.95
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