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Peroxidase catalyzed microbiological oxidation of isosafrol into piperonal

Paper ID Volume ID Publish Year Pages File Format Full-Text
36771 45146 2004 7 PDF Available
Title
Peroxidase catalyzed microbiological oxidation of isosafrol into piperonal
Abstract

A detailed and reliable bioassay to detect peroxidase activity in order to select microbial catalytic agents capable to oxidize isosafrol, 4-(1′-propene)-1,2-methylenedioxybenzene, into piperonal, 4-carboxaldehyde-1,2-methylenedioxibenzene is fully described. The systematic methodology was divided into two parts: first peroxidase activity in microbial systems was detected and determined via the guaiacol assay method and secondly, a biotransformation process to oxidize the substrate was developed. Investigation assays to detect peroxidase activities were performed by using UV-Vis spectrometry, while product formation was monitored by gas chromatography. The biooxidative peroxidase catalyzed transformation of isosafrol into piperonal was achieved with 20% conversion.

Keywords
Microbial screening; Biotransformation; Peroxidase; Oxidation; Isosafrol; Piperonal
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Publisher
Database: Elsevier - ScienceDirect
Journal: Process Biochemistry - Volume 39, Issue 12, 29 October 2004, Pages 2269–2275
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us