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piggyBac-ing models and new therapeutic strategies

Paper ID Volume ID Publish Year Pages File Format Full-Text
36788 45266 2015 9 PDF Available
Title
piggyBac-ing models and new therapeutic strategies
Abstract

•The piggyBac transposon system offers a versatile genome modification system for biotechnology applications.•piggyBac can be used for gene discovery or transgene insertion in vitro or in vivo.•Stable cell lines can be more easily generated with piggyBac as compared with other gene delivery systems.•New therapeutic strategies should result from piggyBac-related gene transfer research.

DNA transposons offer an efficient nonviral method of permanently modifying the genomes of mammalian cells. The piggyBac transposon system has proven effective in genomic engineering of mammalian cells for preclinical applications, including gene discovery, simultaneous multiplexed genome modification, animal transgenesis, gene transfer in vivo achieving long-term gene expression in animals, and the genetic modification of clinically relevant cell types, such as induced pluripotent stem cells and human T lymphocytes. piggyBac has many desirable features, including seamless excision of transposons from the genomic DNA and the potential to target integration events to desired DNA sequences. In this review, we explore these recent applications and also highlight the unique advantages of using piggyBac for developing new molecular therapeutic strategies.

Keywords
piggyBac; transposon; iPSC; transgenesis; cell therapy; immunotherapy
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Publisher
Database: Elsevier - ScienceDirect
Journal: - Volume 33, Issue 9, September 2015, Pages 525–533
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us