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Fine-Tuning Next-Generation Genome Editing Tools

Paper ID Volume ID Publish Year Pages File Format Full-Text
36808 45268 2016 13 PDF Available
Title
Fine-Tuning Next-Generation Genome Editing Tools
Abstract

The availability of genome sequences of numerous organisms and the revolution brought about by genome editing tools (e.g., ZFNs, TALENs, and CRISPR/Cas9 or RGENs) has provided a breakthrough in introducing targeted genetic changes both to explore emergent phenotypes and to introduce new functionalities. However, the wider application of these tools in biology, agriculture, medicine, and biotechnology is limited by off-target mutation effects. In this review, we compare available methods for detecting, measuring, and analyzing off-target mutations. Furthermore, we particularly focus on CRISPR/Cas9 regarding various methods, tweaks, and software tools available to nullify off-target effects.

TrendsThe application of GE tools has significantly influenced medicine, biology, and agriculture for targeted genetic changes to both explore emergent phenotypes and introduce new functionalities with unprecedented control and accuracy.Next-generation GE tools can be optimized to have a method of delivery to cells with high on-target specificity by selecting unique sgRNA or Cas9 variants.Several advanced and optimized methods for unbiased genome-wide profiling of nuclease cleavage sites now provide high-throughput analysis of on- and off-target mutations in single cell-derived clones, humans, animals, and plants.

Keywords
ZFNs; TALENs; CRISPR/Cas9; genome editing; on-target; off-target; target specificity
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Publisher
Database: Elsevier - ScienceDirect
Journal: - Volume 34, Issue 7, July 2016, Pages 562–574
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us