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Redefining Signaling Pathways with an Expanding Single-Cell Toolbox

Paper ID Volume ID Publish Year Pages File Format Full-Text
36816 45269 2016 12 PDF Available
Title
Redefining Signaling Pathways with an Expanding Single-Cell Toolbox
Abstract

Genetically identical cells respond heterogeneously to uniform environmental stimuli. Consequently, investigating the signaling networks that control these cell responses using ‘average’ bulk cell measurements can obscure underlying mechanisms and misses information emerging from cell-to-cell variability. Here we review recent technological advances including live-cell fluorescence imaging-based approaches and microfluidic devices that enable measurements of signaling networks, dynamics, and responses in single cells. We discuss how these single-cell tools have uncovered novel mechanistic insights for canonical signaling pathways that control cell proliferation (ERK), DNA-damage responses (p53), and innate immune and stress responses (NF-κB). Future improvements in throughput and multiplexing, analytical pipelines, and in vivo applicability will all significantly expand the biological information gained from single-cell measurements of signaling pathways.

TrendsMeasuring signaling dynamics in cell populations can obscure underlying mechanisms.New tools are rapidly being developed to measure signaling at single-cell resolution.Single-cell technologies have uncovered fresh mechanistic insights for canonical signaling pathways.Future innovations will increase throughput and multiplexing of single-cell measurements.

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Publisher
Database: Elsevier - ScienceDirect
Journal: - Volume 34, Issue 6, June 2016, Pages 458–469
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us