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Digital detection of biomarkers assisted by nanoparticles: application to diagnostics

Paper ID Volume ID Publish Year Pages File Format Full-Text
36836 45271 2015 9 PDF Available
Title
Digital detection of biomarkers assisted by nanoparticles: application to diagnostics
Abstract

•The new frontier in biomarker analysis is single-molecule counting.•The review reports on techniques allowing digital detection of biomolecules.•The first part focuses on microbead confinement in microenvironments.•The second part describes detection of biological nanoparticles.•Discussion emphasizes clinical applicability.

Single-molecule detection and counting is the new frontier in biomarker analysis. Here, we report on recent techniques for the digital detection of biomolecules for clinical application. First we highlight methods based on the immunocapture of proteins onto microparticles, followed by isolation of individual particles in microenvironments so that a sufficient signal is acquired for each binding event to make a binary decision, thus dramatically enhancing the signal:noise ratio. Various approaches are categorized based on the method used for particle confinement in an isolated microenvironment. We go on to describe methods for the detection of individual biological nanoparticles as well as the digital detection of proteins by artificial nanoparticle labels. The discussion of the methods emphasizes the practical considerations and their clinical applicability.

Keywords
biomarkers; single-molecule; diagnostics; nanoparticle; virus; digital detection; diagnostics
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Publisher
Database: Elsevier - ScienceDirect
Journal: - Volume 33, Issue 6, June 2015, Pages 343–351
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
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