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Operation of a fixed-bed bioreactor in batch and fed-batch modes for production of inulinase by solid-state fermentation

Paper ID Volume ID Publish Year Pages File Format Full-Text
3684 182 2011 11 PDF Available
Title
Operation of a fixed-bed bioreactor in batch and fed-batch modes for production of inulinase by solid-state fermentation
Abstract

This work is focused on the inulinase production by solid-state fermentation (SSF) in a fixed-bed reactor (34 cm diameter and 50 cm height) with working capacity of 2-kg of dry substrate operated in batch and fed-batch modes. It was investigated different strategies for feeding the inlet air in the bioreactor (saturated and unsaturated air) as alternative to remove the metabolic heat generated during the microbial growth by evaporative cooling. The kinetic evaluation of the process carried out in batch mode using unsaturated air showed that the evaporative cooling decreasing the mean temperature of the solid-bed, although the enzyme production was lower than that obtained using saturated air. Results showed that maximum enzyme activity (586 ± 63 U gds−1) was obtained in the fed-batch mode using saturated air after 24 h of fermentation. The enzymatic extract obtained by fed-batch mode was characterized and presented optimum temperature and pH in the range of 52–57 °C and 4.8–5.2, respectively. For a temperature range from 40 to 70 °C the enzyme presented decimal reduction time, D-value, ranging from 5748 to 47 h, respectively. For a pH range from 3.5 to 5.5 the enzyme showed good stability, presenting D-values higher than 2622 h. In terms of Michaelis–Mentem parameters were demonstrated that the crude inulinase activity presented higher affinity for substrate sucrose compared to inulin.

► Evaluation of the performance of inulinases production by SSF using strategies of batch and fed-batch fermentation (FBF). ► Inulinase production in bioreactors pilot scale production and kinetic behavior of the process in terms of temperature and CO2 production. ► The partial characterization of the crude enzymatic extract obtained by FBF was also carried out.

Keywords
Fed-batch fermentation; Agroindustrial residues; Kluyveromyces marxianus NRRL Y-7571; Inulinase; Partial characterization; Solid-state fermentation
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Operation of a fixed-bed bioreactor in batch and fed-batch modes for production of inulinase by solid-state fermentation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volumes 58–59, 15 December 2011, Pages 39–49
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us