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Haploid Strategies for Functional Validation of Plant Genes

Paper ID Volume ID Publish Year Pages File Format Full-Text
36892 45279 2015 10 PDF Available
Title
Haploid Strategies for Functional Validation of Plant Genes
Abstract

Increasing knowledge of plant genome sequences requires the development of more reliable and efficient genetic approaches for genotype–phenotype validation. Functional identification of plant genes is generally achieved by a combination of creating genetic modifications and observing the according phenotype, which begins with forward-genetic methods represented by random physical and chemical mutagenesis and move towards reverse-genetic tools as targeted genome editing. A major bottleneck is time need to produce modified homozygous genotypes that can actually be used for phenotypic validation. Herein, we comprehensively address and compare available experimental approaches for functional validation of plant genes, and propose haploid strategies to reduce the time needed and cost consumed for establishing gene function.

TrendsRecent advances in plant genomics and sequencing technology revealed numerous associations between phenotypes and plant genes. However, definitive functional annotations after in vivo validation have been thoroughly established for few of these genes.Genetic methods for establishing gene functions are achieved by creating genetic modifications that cause phenotypes of interest. Diploid plants are usually heterozygous for the modified region in the first generation. More additional generations are required to obtain homozygous genotypes that can actually be used for validation of their phenotypic effect.Efforts to obtain haploid plants have been undertaken in many plant species of almost all families in the plant kingdom. We proposed the strategies based on haploid mutagenesis and haploid transformation, respectively, which contribute to accelerating functional validation of plant genes.

Keywords
plant genes; functional validation; genetic approaches; haploid mutagenesis; haploid transformation
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Publisher
Database: Elsevier - ScienceDirect
Journal: - Volume 33, Issue 10, October 2015, Pages 611–620
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us