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Optogenetic control of intracellular signaling pathways

Paper ID Volume ID Publish Year Pages File Format Full-Text
36907 45282 2015 9 PDF Available
Title
Optogenetic control of intracellular signaling pathways
Abstract

•We explain mechanisms of light-induced conformational change of photoactivatable proteins.•We describe strategies and studies of using photoactivatable proteins to control intracellular signaling pathways.•We highlight the advantages of using light to control intracellular signaling pathways with superior spatial and temporal resolution.•We discuss precautions to be used in designing experimental schemes of optogenetic control of cell signaling.

Cells employ a plethora of signaling pathways to make their life-and-death decisions. Extensive genetic, biochemical, and physiological studies have led to the accumulation of knowledge about signaling components and their interactions within signaling networks. These conventional approaches, although useful, lack the ability to control the spatial and temporal aspects of signaling processes. The recently emerged optogenetic tools open exciting opportunities by enabling signaling regulation with superior temporal and spatial resolution, easy delivery, rapid reversibility, fewer off-target side effects, and the ability to dissect complex signaling networks. Here we review recent achievements in using light to control intracellular signaling pathways and discuss future prospects for the field, including integration of new genetic approaches into optogenetics.

Keywords
optogenetics; intracellular signaling pathways; signal transduction; light-induced protein–protein interaction; photoactivatable proteins; phytochrome; cryptochrome; LOV; UVR8; Dronpa; oligomerization
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Optogenetic control of intracellular signaling pathways
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Publisher
Database: Elsevier - ScienceDirect
Journal: - Volume 33, Issue 2, February 2015, Pages 92–100
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us