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Enhanced phenolic antioxidants production in submerged cultures of Inonotus obliquus in a ground corn stover medium

Paper ID Volume ID Publish Year Pages File Format Full-Text
3691 182 2011 7 PDF Available
Title
Enhanced phenolic antioxidants production in submerged cultures of Inonotus obliquus in a ground corn stover medium
Abstract

The medicinal mushroom Inonotus obliquus has been a folk remedy for a long time in East-European and Asian countries. It is currently ascribed to a number of phenolic compounds as well as triterpenoids and polysaccharides responsible for significant biological and pharmacological properties. A study was conducted to determine the effects of inclusion of lignocellulosic material, in this case corn stover on production and antioxidant activity of extracellular (EPC) and intracellular phenolic compounds (IPC) by Inonotus obliquus in submerged fermentation. The corn stover medium contained 3% ground corn stover and 3.5% corn flour but the control medium contained 5% corn flour without corn stover. All of the other components were same in the two media. Decomposition rates of cellulose, hemicellulose, and lignin in the corn stover substrate were 20.9%, 17.9%, and 19.8% through 288 h of submerged cultivation. Lignocellulose decomposition in the corn stover-containing medium yielded significantly higher EPC (118.9/135.7 mg GAE (gallic acid equivalents)) and IPC (21.2/23.7 mg GAE) than in the control medium (34.7/42.5 mg GAE of EPC and 12.5/13.5 mg GAE of IPC) per liter of culture broth (EPC) and per gram of mycelia (IPC) in shake flask cultures/10 L fermenter runs. Both EPC and IPC from the corn stover medium showed a higher scavenging activity against hydroxyl radicals and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals than those from the control medium during the later fermentation period. In dose-dependent experiments, EPC from the corn stover medium at 216 h demonstrated a significantly stronger free radical scavenger activity against DPPH and hydroxyl radicals, shown as much lower IC50 values, than that from the control medium and IPC from the two media.

► Extra- and intra-cellular phenols production and antioxidant activity by I. obliquus. ► Effects of lignocellulosic material (corn stover) in shake flask and fermenter cultures. ► A significant increase in phenols production by lignocellulose decomposition. ► Enhanced antioxidant activity of phenols by lignocellulose decomposition.

Keywords
Fermentation; Inonotus obliquus; Microbial growth; Optimisation; Phenolic compounds; Submerged culture
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Enhanced phenolic antioxidants production in submerged cultures of Inonotus obliquus in a ground corn stover medium
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volumes 58–59, 15 December 2011, Pages 103–109
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us