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Quantifying on- and off-target genome editing

Paper ID Volume ID Publish Year Pages File Format Full-Text
36911 45282 2015 9 PDF Available
Title
Quantifying on- and off-target genome editing
Abstract

•Genome editing will transform biomedical research and patient treatment.•Assays for on- and off-target events can be shared across multiple nuclease platforms.•Sequencing-based methods provide the richest data to detect on-target genome editing.•Understanding off-target genome editing requires sequencing and functional assays.

Genome editing with engineered nucleases is a rapidly growing field thanks to transformative technologies that allow researchers to precisely alter genomes for numerous applications including basic research, biotechnology, and human gene therapy. While the ability to make precise and controlled changes at specified sites throughout the genome has grown tremendously in recent years, we still lack a comprehensive and standardized battery of assays for measuring the different genome editing outcomes created at endogenous genomic loci. Here we review the existing assays for quantifying on- and off-target genome editing and describe their utility in advancing the technology. We also highlight unmet assay needs for quantifying on- and off-target genome editing outcomes and discuss their importance for the genome editing field.

Keywords
gene editing; gene targeting; homologous recombination; nonhomologous end-joining; ZFNs; TALENs; CRISPR/Cas9; RNA-guided endonucleases
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Quantifying on- and off-target genome editing
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Publisher
Database: Elsevier - ScienceDirect
Journal: - Volume 33, Issue 2, February 2015, Pages 132–140
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us