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Unnatural amino acid mutagenesis-based enzyme engineering

Paper ID Volume ID Publish Year Pages File Format Full-Text
36919 45284 2015 9 PDF Available
Title
Unnatural amino acid mutagenesis-based enzyme engineering
Abstract

•Enzyme engineering using in vivo incorporation of unnatural amino acids (UAA).•Enhancing and altering enzyme activity using residue- and site-specific incorporation of UAA.•Enzyme evolution with UAA by integrating directed evolution and UAA incorporation.

Traditional enzyme engineering relies on substituting one amino acid by one of the other 19 natural amino acids to change the functional properties of an enzyme. However, incorporation of unnatural amino acids (UAAs) has been harnessed to engineer efficient enzymes for biocatalysis. Residue-specific and site-specific in vivo incorporation methods are becoming the preferred approach for producing enzymes with altered or improved functions. We describe the contribution of in vivo UAA incorporation methodologies to enzyme engineering as well as the future prospects for the field, including the integration of UAAs with other new advances in enzyme engineering.

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Unnatural amino acid mutagenesis-based enzyme engineering
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Publisher
Database: Elsevier - ScienceDirect
Journal: - Volume 33, Issue 8, August 2015, Pages 462–470
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
Online Support
Any Questions? feel free to contact us